Constitutive and differential expression of transport protein genes in Parascaris univalens larvae and adult tissues after in vitro exposure to anthelmintic drugs

• Development of a novel hatching protocol for P. univalens eggs. Hatching rate 92 %. Lower expression several transport protein genes in larvae than adult tissues. Up-regulation pgp-9 vitro exposed to pyrantel and thiabendazole. No up-regulation tissues after drug exposure. The equine roundworm Parascaris has developed resistance the three anthelmintic substances most commonly used horses. mechanisms responsible are believed be multi-genic, proteins such as P-glycoprotein (Pgp) family have been suggested involved parasites including univlaens . To facilitate further research into behind metabolism development we aimed develop an model based on larvae. We fast easy studies, resulting pgp-2 , pgp-11.1 pgp-16.1 major facilitator superfamily (MFS) PgR006_g137 PgR015_g078 were studied hatched drugs ivermecin (IVM) 10 −9 M, citrate (PYR) −6 M thiabendazole (TBZ) −5 24 h. In comparison, these was anterior end intestinal worms IVM, TBZ PYR, at same concentrations larvae, 3 h, h Larval exposure sub-lethal doses IVM did not affect levels any investigated genes, however PYR showed significantly increased result significant increases genes. Comparisons constitutive between worm that MFS gene expressed lower tissues, while had similar worms. All higher rates intestine worms, except where two This high suggests this is important site xenobiotic efflux Despite fact results study show differences believe larval assay system described here will tool molecular other studies.